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trap solution in trap stain kit  (Beijing Solarbio Science)


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    Beijing Solarbio Science trap solution in trap stain kit
    Trap Solution In Trap Stain Kit, supplied by Beijing Solarbio Science, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/trap solution in trap stain kit/product/Beijing Solarbio Science
    Average 90 stars, based on 1 article reviews
    trap solution in trap stain kit - by Bioz Stars, 2026-06
    90/100 stars

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    Denosumab inhibits MCF-7-induced spontaneous osteoclastogenesis of RAW 264.7 cells. (A) Co-culture system for spontaneous osteoclastogenesis of RAW 264.7 cells induced by MCF-7 cells; (B) representative field of co-culture after <t>TRAP</t> <t>staining</t> and statistics of TRAP+ osteoclastogenesis; (C,D) the mRNA expression of bone resorption gene (C) and osteoclast phenotype gene (D). Three times for each experiment and ***, P<0.001 vs . control group. Scale bar =20 mm. TRAP, tartrate-resistant acid phosphatase.
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    Denosumab inhibits MCF-7-induced spontaneous osteoclastogenesis of RAW 264.7 cells. (A) Co-culture system for spontaneous osteoclastogenesis of RAW 264.7 cells induced by MCF-7 cells; (B) representative field of co-culture after <t>TRAP</t> <t>staining</t> and statistics of TRAP+ osteoclastogenesis; (C,D) the mRNA expression of bone resorption gene (C) and osteoclast phenotype gene (D). Three times for each experiment and ***, P<0.001 vs . control group. Scale bar =20 mm. TRAP, tartrate-resistant acid phosphatase.
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    Three-month-old WT and Gsdmd -/- male mice were left untreated or subjected to 9-Gy TBI. Irradiated mice were transplanted with 10 7 bone marrow cells from 3-month-old WT or Gsdmd -/- male mice to generate WT→WT, Gsdmd -/- →WT, WT→ Gsdmd -/- , and Gsdmd -/- → Gsdmd -/- mice. Samples were harvested 3 weeks later. The femurs were analyzed by μCT. (A, C) Cross sections of 3D reconstructions. (B, D) BV/TV. The femurs were also stained for TRAP activity. (E) TRAP + cells (OCs), stained in red. (F) N.Oc/BS. Scale bar: 200 μm. The percentage of OC progenitors (“prog”) in bone marrow cells was analyzed by flow cytometry (G; the gating is shown in ). (H) H&E staining of the spleen. Scale bar: 100 μm. (I) Percentage of spleen weights. The numerical values underlying Fig 2B, 2D, 2F, 2G, 2I can be found in . Data are mean ± SEM. * P < 0.05, ** P < 0.005, *** P < 0.0005, **** P < 0.0001. μCT, micro–computed tomography; BV/TV, bone volume/total volume; GSDMD, gasdermin D; H&E, hematoxylin and eosin; N.Oc/BS, OC number/bone surface; ns, not significant; OC, osteoclast; TBI, total body irradiation; WT, wild-type.

    Journal: PLoS Biology

    Article Title: Radiation causes tissue damage by dysregulating inflammasome–gasdermin D signaling in both host and transplanted cells

    doi: 10.1371/journal.pbio.3000807

    Figure Lengend Snippet: Three-month-old WT and Gsdmd -/- male mice were left untreated or subjected to 9-Gy TBI. Irradiated mice were transplanted with 10 7 bone marrow cells from 3-month-old WT or Gsdmd -/- male mice to generate WT→WT, Gsdmd -/- →WT, WT→ Gsdmd -/- , and Gsdmd -/- → Gsdmd -/- mice. Samples were harvested 3 weeks later. The femurs were analyzed by μCT. (A, C) Cross sections of 3D reconstructions. (B, D) BV/TV. The femurs were also stained for TRAP activity. (E) TRAP + cells (OCs), stained in red. (F) N.Oc/BS. Scale bar: 200 μm. The percentage of OC progenitors (“prog”) in bone marrow cells was analyzed by flow cytometry (G; the gating is shown in ). (H) H&E staining of the spleen. Scale bar: 100 μm. (I) Percentage of spleen weights. The numerical values underlying Fig 2B, 2D, 2F, 2G, 2I can be found in . Data are mean ± SEM. * P < 0.05, ** P < 0.005, *** P < 0.0005, **** P < 0.0001. μCT, micro–computed tomography; BV/TV, bone volume/total volume; GSDMD, gasdermin D; H&E, hematoxylin and eosin; N.Oc/BS, OC number/bone surface; ns, not significant; OC, osteoclast; TBI, total body irradiation; WT, wild-type.

    Article Snippet: At the end of the culture period, the cells were rinsed with water and incubated with the TRAP staining solution (Sigma leukocyte acid phosphatase kit) at room temperature for 30 minutes.

    Techniques: Irradiation, Staining, Activity Assay, Flow Cytometry, Micro-CT

    Denosumab inhibits MCF-7-induced spontaneous osteoclastogenesis of RAW 264.7 cells. (A) Co-culture system for spontaneous osteoclastogenesis of RAW 264.7 cells induced by MCF-7 cells; (B) representative field of co-culture after TRAP staining and statistics of TRAP+ osteoclastogenesis; (C,D) the mRNA expression of bone resorption gene (C) and osteoclast phenotype gene (D). Three times for each experiment and ***, P<0.001 vs . control group. Scale bar =20 mm. TRAP, tartrate-resistant acid phosphatase.

    Journal: Translational Cancer Research

    Article Title: Denosumab inhibits MCF-7 cell line-induced spontaneous osteoclastogenesis via the RANKL/MALAT1/miR-124 axis

    doi: 10.21037/tcr.2020.03.17

    Figure Lengend Snippet: Denosumab inhibits MCF-7-induced spontaneous osteoclastogenesis of RAW 264.7 cells. (A) Co-culture system for spontaneous osteoclastogenesis of RAW 264.7 cells induced by MCF-7 cells; (B) representative field of co-culture after TRAP staining and statistics of TRAP+ osteoclastogenesis; (C,D) the mRNA expression of bone resorption gene (C) and osteoclast phenotype gene (D). Three times for each experiment and ***, P<0.001 vs . control group. Scale bar =20 mm. TRAP, tartrate-resistant acid phosphatase.

    Article Snippet: Then, the cells were cytospun onto slides, and TRAP staining was performed using a TRAP staining solution kit (Solarbio, G1942, China).

    Techniques: Co-Culture Assay, Staining, Expressing, Control

    MALAT1 regulates spontaneous osteoclastogenesis of RAW 264.7 cells inhibited by denosumab. (A) Representative field of co-culture after TRAP staining and statistics of TRAP+ osteoclastogenesis; (B) MALAT expressed in each group; (C) immunofluorescence staining of RANKL in RAW 264.7 cells of each group. Three times for each experiment; ***, P<0.001 vs. control group, and ### , P<0.001 vs. denosumab group. Scale bar =50 µm. TRAP, tartrate-resistant acid phosphatase; RANKL, receptor activator of NF-κB ligand.

    Journal: Translational Cancer Research

    Article Title: Denosumab inhibits MCF-7 cell line-induced spontaneous osteoclastogenesis via the RANKL/MALAT1/miR-124 axis

    doi: 10.21037/tcr.2020.03.17

    Figure Lengend Snippet: MALAT1 regulates spontaneous osteoclastogenesis of RAW 264.7 cells inhibited by denosumab. (A) Representative field of co-culture after TRAP staining and statistics of TRAP+ osteoclastogenesis; (B) MALAT expressed in each group; (C) immunofluorescence staining of RANKL in RAW 264.7 cells of each group. Three times for each experiment; ***, P<0.001 vs. control group, and ### , P<0.001 vs. denosumab group. Scale bar =50 µm. TRAP, tartrate-resistant acid phosphatase; RANKL, receptor activator of NF-κB ligand.

    Article Snippet: Then, the cells were cytospun onto slides, and TRAP staining was performed using a TRAP staining solution kit (Solarbio, G1942, China).

    Techniques: Co-Culture Assay, Staining, Immunofluorescence, Control

    miR-124 regulates spontaneous osteoclastogenesis of RAW 264.7 cells inhibited by denosumab. (A) Representative field of co-culture after TRAP staining and statistics of TRAP+ osteoclastogenesis; (B) miR-124 expressed in each group; (C) immunofluorescence staining of RANKL in RAW 264.7 cells of each group. Three times for each experiment, ***, P<0.001 vs . control group, and ### , P<0.001 vs. denosumab group. Scale bar =50 µm. TRAP, tartrate-resistant acid phosphatase; RANKL, receptor activator of NF-κB ligand.

    Journal: Translational Cancer Research

    Article Title: Denosumab inhibits MCF-7 cell line-induced spontaneous osteoclastogenesis via the RANKL/MALAT1/miR-124 axis

    doi: 10.21037/tcr.2020.03.17

    Figure Lengend Snippet: miR-124 regulates spontaneous osteoclastogenesis of RAW 264.7 cells inhibited by denosumab. (A) Representative field of co-culture after TRAP staining and statistics of TRAP+ osteoclastogenesis; (B) miR-124 expressed in each group; (C) immunofluorescence staining of RANKL in RAW 264.7 cells of each group. Three times for each experiment, ***, P<0.001 vs . control group, and ### , P<0.001 vs. denosumab group. Scale bar =50 µm. TRAP, tartrate-resistant acid phosphatase; RANKL, receptor activator of NF-κB ligand.

    Article Snippet: Then, the cells were cytospun onto slides, and TRAP staining was performed using a TRAP staining solution kit (Solarbio, G1942, China).

    Techniques: Co-Culture Assay, Staining, Immunofluorescence, Control